Tietz Video and Image Processing Systems GmbH

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Automatic TEM alignment and acquisition of tomographic tilt series
In cooperation with the Max-Planck-Institute for Biochemistry in Martinsried, TVIPS GmbH has developed a software solution for automatic TEM alignment and acquisition of tomographic tilt series which is compatible with nearly all TEMs equipped with remote control and computer-controlled goniometer. This system includes tools for automatic focusing and correction of astigmatism. A tracking routine compensates for tilt or drift induced image shifts for each projection. A low dose utility for all alignment procedures minimizes the electron dose applied to the specimen as all corrections can be performed on adjacent areas. Thus, the region of interest is merely exposed to the dose intended for the image acquisition.

Features and benefits

Automatic acquisition of tomographic tilt series under low dose conditions
Compensation of tilt-induced specimen displacement using image and beam shift
Integrated auto-focus
Detail centering in an overview image
Sophisticated low dose features defining up to 5 imaging areas on the specimen with independent and automatically stored settings of magnification, spot size, exposure time, image and beam shift and camera parameters (size and binning factor).
Image area/resolution can be increased by tiling
Continuously updated display of applied electron dose
Turn key solutions are available for FEI/Philips, Hitachi, JEOL, and LEO microscopes
Typical execution time for one tilt step, including displacement and focus correction (depending on TEM type): 15 - 120 sec