For high-end scientific imaging in Transmission Electron Microscopy, TVIPS were the first to design a new generation of CMOS based TEM cameras with active pixel sensors available in three different resolutions: TemCam-F816 (8k × 8k), TemCam-F416 (4k × 4k) and TemCam-F216 (2k × 2k).


TemCam8kTemCam-F816 is the first digital camera with a larger image area than standard film. With its impressive area of 128 × 128 mm² and ultra-high resolution of 64 megapixel the F816 surpasses the performance of photo plates. This camera opens up extraordinary possibilities for high throughput applications such as single particle data collection or screening of sections which require rapid acquisition of very large, high-resolution data sets. Since CMOS sensors show absolutely no blooming and have a high dynamic range they are well suited for acquisition of diffraction patterns. Now the second generation of F816 incorporates the latest 16 bit electronics.

4kTemCam-F416 & F416R (Retractable, placed in JEOL photo chamber) are 16 megapixel cameras covering an image area of 64 × 64 mm². This camera type, with its unique combination of high sensitivity, exceptional acquisition speed and a huge dynamic range, has replaced classical 4k slow scan CCD cameras.

2kTemCam-F216 is TVIPS latest 4 megapixel camera, covering an image area of 32 × 32 mm². Having the same performance as the F816 and F416 with smaller sensor area, this camera is an ideal solution for limited budgets. The field of view can be extended by automatic software tiling.

Fast Readout Fast Readout

The readout is done simultaneously on 8, 4, or 2 channels respectively with 10 MHz. The typical readout time of a 4k × 4k area is 1 second, which represents an improvement of around 5× in comparison to up-to-date 4k multi-port CCD cameras. For a subarea of 2k × 2k, frame rates of 1.8 fps can be achieved.

Rolling Shutter Mode Rolling Shutter Mode

For even faster readout the sensor can be operated in the rolling shutter mode whereby no beam shutter is needed. In this mode, real time observation and data storage with frame rates of 8.5 fps on sub areas as large as 8k × 1k can be achieved.

Optimized Scintillator Optimized Scintillator

TVIPS optimizes the scintillator for individual demands. For high tension ranging from 40 kV – 60kV up to 400 kV, two standard types are available: optimized for high resolution or for high sensitivity. On customer request, individual scintillators can be manufactured.

Fiber-Optical Coupling Fiber-Optical Coupling

Fiber-optical coupling of the electron-sensitive layer (scintillator) with the sensor increases the amount of light collected in comparison to lens-optical coupling and, as a result, the sensitivity of the camera.

Sensor Cooling Sensor Cooling

For optimum performance, the Peltier-cooled CMOS sensor is located in a vacuum housing separated from the microscope vacuum. The scintillator surface is kept at room temperature. This design avoids contamination and allows the venting of the camera chamber without warming up the CMOS sensor.

Near-Axis Flange Near-Axis Flange

TVIPS has designed a special flange which optionally combines the TemCam-F816/TemCam-F416 with the FastScan-F114NX, a high-quality CCD camera operating at video rate (up to 30 fps). This camera can be used for real-time applications such as alignment, screening of the sample and in situ experiments.

SPECIFICATIONS TemCam-F216 TemCam-F416 (R) TemCam-F816
* Typical Values
Sensor type CMOS
Format 2048 × 2048 4096 × 4096 8192 × 8192
Pixel size (µm2) 15.6 × 15.6 µm2
Fill factor 72%
Field of view 31.9 × 31.9 mm2 63.9 × 63.9 mm2 127.8 × 127.8 mm2
Readout rate (16 bit) 2 × 10 megapixel/sec 4 × 10 megapixel/sec 8 × 10 megapixel/sec
Frame rates: – 1.8 fps, full resolution, 1× binning

– 3.0 fps, subarea 1k × 1k, 1× binning

– 4.5 fps, full area, 2k × 2k, RS

– 8.5 fps, subarea, 1k × 1k, RS

– 8.5 fps, subarea, 2k × 1k, RS

– 1.0 fps, full resolution, 1× binning

– 1.8 fps, subarea, 2k × 2k, 1× binning

– 2.5 fps, subarea 4k × 1k, 1× binning

– 2.5 fps, full area, 2× binning, RS

– 4.5 fps, subarea, 2k × 2k, RS

– 8.5 fps, subarea, 1k × 1k, RS

– 8.5 fps, subarea, 4k × 1k, RS

– 0.4 fps, full resolution, 1× binning

– 1.8 fps, subarea2k × 2k, 1× binning

– 2.5 fps, subarea8k × 1k, 1× binning

– 1.2 fps, full area, 2× binning, RS

– 4.5 fps, subarea, 2k × 2k, RS

– 8.5 fps, subarea, 1k × 1k, RS

– 8.5 fps, subarea, 8k × 1k, RS

Post magnification ~1.1× 1.4× – 1.8× 1.8× – 3.5×
Electron-optical coupling 1:1 fiber optics
Scintillator type polycrystalline phosphor
Sensor cooling thermoelectric
Binning factors 1×, 2×, 4×
Full well capacity (CCD electrons) 170 000
Dynamic range (maximum/noise) 10.000 : 1
(after flatfield correction)
< 1%
SNR (signal-to-noise-ratio)
for single electrons
~14:1 (120 kV)
~12:1 (200 kV)
~14:1 (120 kV)
~12:1 (200 kV)
~10:1 (120 kV)
~8:1 (200 kV)
Resolution @ 200 kV
(NTF at Nyquist frequency)
~14% ~11% ~10%
Anti blooming blooming not present
Bottom mounted on-axis on-axis, rotatable on-axis
System requirements Windows XP and 7
Intel DualCore CPU
Optical Gigabit Ethernet
PCI Express ×4 slot
Windows XP and 7
Intel DualCore CPU
Optical 10 Gigabit Ethernet
PCI Express ×4 slot
Options FastScan-F114NX for FEI, FastScan-114TR for JEOL
Software EM-MENU, Tomography, Single Particle Collection, EM-SPECTRO, recording to hard disk